Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

FAF1 phosphorylation by AKT accumulates TGF-Î² type II receptor and drives breast cancer metastasis.

Xie F, Jin K, Shao L, Fan Y, Tu Y, Li Y, Yang B, van Dam H, Ten Dijke P, Weng H, Dooley S, Wang S, Jia J, Jin J, Zhou F, Zhang L

TGF-Î² is pro-metastatic for the late-stage breast cancer cells. Despite recent progress, the regulation of TGF-Î² type II receptor remains uncertain. Here we report that FAF1 destabilizes TÎ²RII on the cell surface by recruiting the VCP/E3 ligase complex, thereby limiting excessive TGF-Î² response. Importantly, activated AKT directly phosphorylates FAF1 at Ser 582, which disrupts the FAF1-VCP complex and reduces FAF1 ... [more]

Nat Commun Dec. 26, 2016; 8();15021 [Pubmed: 28443643]

Throughput

Low Throughput

Additional Notes

The overexpression or deletion of FAF1 caused measurable changes (sometimes more, sometimes less, see paper for details) of all the hit proteins listed at the cell membrane as assayed by streptavidin pull-downs of biotin-labeled cell surfaces: VCP, UFD1, NPL4, betaTRCP, CUL1, and TbetaRII.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
VCP FAF1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Alexandru G (2008)	Low	-	BioGRID	-
FAF1 VCP	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9881	BioGRID	3084892
FAF1 VCP	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lee JJ (2013)	Low	-	BioGRID	-
FAF1 VCP	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Raman M (2015)	High	-	BioGRID	-
FAF1 VCP	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Song S (2022)	Low	-	BioGRID	-
FAF1 VCP	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Xie F (2017)	High	-	BioGRID	-
VCP FAF1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Yu CC (2013)	High	-	BioGRID	817290
FAF1 VCP	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Alexandru G (2008)	Low	-	BioGRID	-
FAF1 VCP	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Alexandru G (2012)	Low	-	BioGRID	728188
FAF1 VCP	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Baron Y (2014)	Low	-	BioGRID	-
FAF1 VCP	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Franz A (2016)	Low	-	BioGRID	-
FAF1 VCP	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Haenzelmann P (2011)	Low	-	BioGRID	-
FAF1 VCP	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Lee JJ (2013)	Low	-	BioGRID	-
FAF1 VCP	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Song EJ (2005)	Low	-	BioGRID	-
VCP FAF1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Xie F (2017)	Low	-	BioGRID	-
FAF1 VCP	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Xie F (2017)	Low	-	BioGRID	-
FAF1 VCP	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Haenzelmann P (2011)	Low	-	BioGRID	-
VCP FAF1	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Kang W (2011)	Low	-	BioGRID	-
VCP FAF1	Co-crystal Structure Co-crystal Structure Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.	Shin HY (2010)	Low	-	BioGRID	-
FAF1 VCP	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Haenzelmann P (2011)	Low	-	BioGRID	-
VCP FAF1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Lee JJ (2013)	Low	-	BioGRID	-
VCP FAF1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Song EJ (2005)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

FAF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NF-kappaB binding [IPI]heat shock protein binding [IDA]protein binding [IPI]protein kinase binding [IDA]protein kinase regulator activity [NAS]ubiquitin binding [IDA]ubiquitin protein ligase binding [IDA]

Gene Ontology Cellular Component

VCP

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [TAS]deubiquitinase activator activity [IDA]poly(A) RNA binding [IDA]polyubiquitin binding [IDA]protein binding [IPI]protein domain specific binding [IPI]protein phosphatase binding [IPI]ubiquitin-specific protease binding [IPI]