BAIT

CLA4

ERC10, serine/threonine protein kinase CLA4, L000000564, L000002643, YNL298W
Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

RTT107

ESC4, L000004424, YHR154W
Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
GO Process (2)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Mapping DNA damage-dependent genetic interactions in yeast via party mating and barcode fusion genetics.

Diaz-Mejia JJ, Celaj A, Mellor JC, Cote A, Balint A, Ho B, Bansal P, Shaeri F, Gebbia M, Weile J, Verby M, Karkhanina A, Zhang Y, Wong C, Rich J, Prendergast D, Gupta G, Oeztuerk S, Durocher D, Brown GW, Roth FP

Condition-dependent genetic interactions can reveal functional relationships between genes that are not evident under standard culture conditions. State-of-the-art yeast genetic interaction mapping, which relies on robotic manipulation of arrays of double-mutant strains, does not scale readily to multi-condition studies. Here, we describe barcode fusion genetics to map genetic interactions (BFG-GI), by which double-mutant strains generated via en masse "party" mating ... [more]

Mol. Syst. Biol. Dec. 28, 2017; 14(5);e7985 [Pubmed: 29807908]

Quantitative Score

  • -0.399148804 [Confidence Score]

Throughput

  • High Throughput

Ontology Terms

  • vegetative growth (APO:0000106)

Additional Notes

  • Cultures grown in MMS ((CHEBI:25255))
  • Interactions determined by barcode fusion genetics to map genetic interactions (BFG-GI) using a ZGenetic Interaction Score (GIS)cutoff corresponding to FDR=0.01 and an additional effect?size cutoff (-0.075>GIS>0.075)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RTT107 CLA4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Low-0.2673BioGRID
560536
RTT107 CLA4
Synthetic Growth Defect
Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

High-BioGRID
453351

Curated By

  • BioGRID