Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Schizosaccharomyces pombe protein phosphatase 1 in mitosis, endocytosis and a partnership with Wsh3/Tea4 to control polarised growth.

Alvarez-Tabares I, Grallert A, Ortiz JM, Hagan IM

PP1 holoenzymes are composed of a small number of catalytic subunits and an array of regulatory, targeting, subunits. The Schizosaccharomyces pombe genome encodes two highly related catalytic subunits, Dis2 and Sds21. The gene for either protein can be individually deleted, however, simultaneous deletion of both is lethal. We fused enhanced green fluorescent protein (EGFP) coding sequences to the 5' end ... [more]

J. Cell. Sci. Oct. 15, 2007; 120(0);3589-601 [Pubmed: 17895368]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DIS2 SDS21	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Kinoshita N (1990)	Low	-	BioGRID	247622
DIS2 SDS21	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Ohkura H (1989)	Low	-	BioGRID	300800

Curated By

BioGRID

Interaction Summary

DIS2

Gene Ontology Biological Process

Gene Ontology Molecular Function

phosphoprotein phosphatase activity [IDA]protein serine/threonine phosphatase activity [IDA]

Gene Ontology Cellular Component

SDS21