An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis.

Capalbo L, Bassi ZI, Geymonat M, Todesca S, Copoiu L, Enright AJ, Callaini G, Riparbelli MG, Yu L, Choudhary JS, Ferrero E, Wheatley S, Douglas ME, Mishima M, D'Avino PP

The midbody is an organelle assembled at the intercellular bridge between the two daughter cells at the end of mitosis. It controls the final separation of the daughter cells and has been involved in cell fate, polarity, tissue organization, and cilium and lumen formation. Here, we report the characterization of the intricate midbody protein-protein interaction network (interactome), which identifies many ... [more]

Unknown Dec. 04, 2018; 10(1);4513 [Pubmed: 31586073]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AURKB CCT7	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Buljan M (2020)	High	31.3158	BioGRID	3485865

Curated By

BioGRID

Interaction Summary

AURKB

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone serine kinase activity [IBA, ISS]protein binding [IPI]protein serine/threonine kinase activity [IDA]protein serine/threonine/tyrosine kinase activity [TAS]

Gene Ontology Cellular Component

CCT7