BAIT

APC

BTPS2, DP2, DP2.5, DP3, GS, PPP1R46
adenomatous polyposis coli
GO Process (21)
GO Function (8)
GO Component (12)
Homo sapiens
PREY

PIK3CA

CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
Homo sapiens

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Network-Based Combinatorial CRISPR-Cas9 Screens Identify Synergistic Modules in Human Cells.

Guo Y, Bao C, Ma D, Cao Y, Li Y, Xie Z, Li S

Tumorigenesis is a complex process that is driven by a combination of networks of genes and environmental factors; however, efficient approaches to identifying functional networks that are perturbed by the process of tumorigenesis are lacking. In this study, we provide a comprehensive network-based strategy for the systematic discovery of functional synergistic modules that are causal determinants of inflammation-induced tumorigenesis. Our ... [more]

ACS Synth Biol Dec. 15, 2018; 8(3);482-490 [Pubmed: 30762338]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: growth abnormality (HP:0001507)
  • phenotype: viability (PATO:0000169)

Additional Notes

  • CRISPR GI screen
  • Cell Line:NCM460 cells
  • Experimental Setup: Cytokine Exposure (TGFB1)
  • GIST: A-phenotypic negative genetic interaction
  • Library: Targeted Library
  • Significance Threshold: dGI < -0.84

Curated By

  • BioGRID