BAIT

KIN-20

CELE_F46F2.2
Protein KIN-20
Caenorhabditis elegans
PREY

HIM-6

CELE_T04A11.6, blm-1, T04A11.6
him-6 encodes a RecQ-like ATP-dependent DNA helicase orthologous to human BLM (OMIM:604610, mutated in Bloom syndrome); HIM-6 is required for normal levels of recombination during meiosis and as such, loss of HIM-6 function results in an increased frequency of genotypically XO males and inviable aneuoploid embryos as a result of higher levels of chromosome nondisjunction; HIM-6 interacts both genetically and in vitro with TOP-3, DNA topoisomerase IIIalpha, with which it functions to regulate genomic stability during germ-line mitosis; HIM-6 is expressed primarily in the germ line.
Caenorhabditis elegans

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

C. elegans ORFeome version 1.1: experimental verification of the genome annotation and resource for proteome-scale protein expression.

Reboul J, Vaglio P, Rual JF, Lamesch P, Martinez M, Armstrong CM, Li S, Jacotot L, Bertin N, Janky R, Moore T, Hudson JR, Hartley JL, Brasch MA, Vandenhaute J, Boulton S, Endress GA, Jenna S, Chevet E, Papasotiropoulos V, Tolias PP, Ptacek J, Snyder M, Huang R, Chance MR, Lee H, Doucette-Stamm L, Hill DE, Vidal M

To verify the genome annotation and to create a resource to functionally characterize the proteome, we attempted to Gateway-clone all predicted protein-encoding open reading frames (ORFs), or the 'ORFeome,' of Caenorhabditis elegans. We successfully cloned approximately 12,000 ORFs (ORFeome 1.1), of which roughly 4,000 correspond to genes that are untouched by any cDNA or expressed-sequence tag (EST). More than 50% ... [more]

Nat. Genet. May. 01, 2003; 34(1);35-41 [Pubmed: 12679813]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KIN-20 HIM-6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
KIN-20 HIM-6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
HIM-6 KIN-20
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-WormBase
-

Curated By