BAIT

KIN-20

CELE_F46F2.2
Protein KIN-20
Caenorhabditis elegans
PREY

HIM-6

CELE_T04A11.6, blm-1, T04A11.6
him-6 encodes a RecQ-like ATP-dependent DNA helicase orthologous to human BLM (OMIM:604610, mutated in Bloom syndrome); HIM-6 is required for normal levels of recombination during meiosis and as such, loss of HIM-6 function results in an increased frequency of genotypically XO males and inviable aneuoploid embryos as a result of higher levels of chromosome nondisjunction; HIM-6 interacts both genetically and in vitro with TOP-3, DNA topoisomerase IIIalpha, with which it functions to regulate genomic stability during germ-line mitosis; HIM-6 is expressed primarily in the germ line.
Caenorhabditis elegans

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A map of the interactome network of the metazoan C. elegans.

Li S, Armstrong CM, Bertin N, Ge H, Milstein S, Boxem M, Vidalain PO, Han JD, Chesneau A, Hao T, Goldberg DS, Li N, Martinez M, Rual JF, Lamesch P, Xu L, Tewari M, Wong SL, Zhang LV, Berriz GF, Jacotot L, Vaglio P, Reboul J, Hirozane-Kishikawa T, Li Q, Gabel HW, Elewa A, Baumgartner B, Rose DJ, Yu H, Bosak S, Sequerra R, Fraser A, Mango SE, Saxton WM, Strome S, Van Den Heuvel S, Piano F, Vandenhaute J, Sardet C, Gerstein M, Doucette-Stamm L, Gunsalus KC, Harper JW, Cusick ME, Roth FP, Hill DE, Vidal M

To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together ... [more]

Science Jan. 23, 2004; 303(5657);540-3 [Pubmed: 14704431]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KIN-20 HIM-6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
HIM-6 KIN-20
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-WormBase
-
KIN-20 HIM-6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-WormBase
-

Curated By

  • BioGRID