BAIT

KIN-20

CELE_F46F2.2
Protein KIN-20
Caenorhabditis elegans
PREY

HIM-6

CELE_T04A11.6, blm-1, T04A11.6
him-6 encodes a RecQ-like ATP-dependent DNA helicase orthologous to human BLM (OMIM:604610, mutated in Bloom syndrome); HIM-6 is required for normal levels of recombination during meiosis and as such, loss of HIM-6 function results in an increased frequency of genotypically XO males and inviable aneuoploid embryos as a result of higher levels of chromosome nondisjunction; HIM-6 interacts both genetically and in vitro with TOP-3, DNA topoisomerase IIIalpha, with which it functions to regulate genomic stability during germ-line mitosis; HIM-6 is expressed primarily in the germ line.
Caenorhabditis elegans

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Combined functional genomic maps of the C. elegans DNA damage response.

Boulton SJ, Gartner A, Reboul J, Vaglio P, Dyson N, Hill DE, Vidal M

Many human cancers originate from defects in the DNA damage response (DDR). Although much is known about this process, it is likely that additional DDR genes remain to be discovered. To identify such genes, we used a strategy that combines protein-protein interaction mapping and large-scale phenotypic analysis in Caenorhabditis elegans. Together, these approaches identified 12 worm DDR orthologs and 11 ... [more]

Science Jan. 04, 2002; 295(5552);127-31 [Pubmed: 11778048]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KIN-20 HIM-6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
HIM-6 KIN-20
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-WormBase
-
KIN-20 HIM-6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-WormBase
-

Curated By

  • BioGRID