BAIT

NPC1

NPC
Niemann-Pick disease, type C1
Homo sapiens
PREY

LAMTOR1

C11orf59, PDRO, Ragulator1, p18, p27RF-Rho, PP7157
late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export.

van den Boomen DJH, Sienkiewicz A, Berlin I, Jongsma MLM, van Elsland DM, Luzio JP, Neefjes JJC, Lehner PJ

Cholesterol import in mammalian cells is mediated by the LDL receptor pathway. Here, we perform a genome-wide CRISPR screen using an endogenous cholesterol reporter and identify >100 genes involved in LDL-cholesterol import. We characterise C18orf8 as a core subunit of the mammalian Mon1-Ccz1 guanidine exchange factor (GEF) for Rab7, required for complex stability and function. C18orf8-deficient cells lack Rab7 activation ... [more]

Nat Commun Dec. 03, 2019; 11(1);5559 [Pubmed: 33144569]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LAMTOR1 NPC1
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High40BioGRID
2993865

Curated By

  • BioGRID