An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export.

van den Boomen DJH, Sienkiewicz A, Berlin I, Jongsma MLM, van Elsland DM, Luzio JP, Neefjes JJC, Lehner PJ

Cholesterol import in mammalian cells is mediated by the LDL receptor pathway. Here, we perform a genome-wide CRISPR screen using an endogenous cholesterol reporter and identify >100 genes involved in LDL-cholesterol import. We characterise C18orf8 as a core subunit of the mammalian Mon1-Ccz1 guanidine exchange factor (GEF) for Rab7, required for complex stability and function. C18orf8-deficient cells lack Rab7 activation ... [more]

Nat Commun Dec. 03, 2019; 11(1);5559 [Pubmed: 33144569]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SIGMAR1 NPC1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9654	BioGRID	3168883

Curated By

BioGRID

Interaction Summary

NPC1

Gene Ontology Biological Process

Gene Ontology Molecular Function

cholesterol binding [IDA]protein binding [IPI]receptor activity [TAS]sterol transporter activity [TAS]transmembrane signaling receptor activity [TAS]

Gene Ontology Cellular Component

SIGMAR1