PREY

CDK1

CDC2, CDC28A, P34CDC2
cyclin-dependent kinase 1
GO Process (46)
GO Function (5)
GO Component (11)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

FEZ1/LZTS1 gene at 8p22 suppresses cancer cell growth and regulates mitosis.

Ishii H, Vecchione A, Murakumo Y, Baldassarre G, Numata S, Trapasso F, Alder H, Baffa R, Croce CM

The FEZ1/LZTS1 gene maps to chromosome 8p22, a region that is frequently deleted in human tumors. Alterations in FEZ1/LZTS1 expression have been observed in esophageal, breast, and prostate cancers. Here, we show that introduction of FEZ1/LZTS1 into Fez1/Lzts1-negative cancer cells results in suppression of tumorigenicity and reduced cell growth with accumulation of cells at late S-G(2)/M stage of the cell ... [more]

Proc. Natl. Acad. Sci. U.S.A. Aug. 28, 2001; 98(18);10374-9 [Pubmed: 11504921]

Throughput

  • Low Throughput

Curated By

  • BioGRID