An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry.

Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI

Recently, we identified proteins that co-purify with the human spliceosome using mass spectrometry. One of the identified proteins, CDC5L, corresponds to the human homologue of the Schizosaccharomyces pombe CDC5(+) gene product. Here we show that CDC5L is part of a larger multiprotein complex in HeLa nuclear extract that incorporates into the spliceosome in an ATP-dependent step. We also show that ... [more]

EMBO J. Dec. 01, 2000; 19(23);6569-81 [Pubmed: 11101529]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CDC5L SCAF8	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Lleres D (2010)	High	-	BioGRID	-
CDC5L SCAF8	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Ajuh P (2000)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CDC5L

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [NAS]WD40-repeat domain binding [IDA]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

SCAF8