SKPA
Gene Ontology Biological Process
- DNA endoreduplication [IMP]
- centrosome duplication [IMP]
- chromosome condensation [IMP]
- mitotic G2 DNA damage checkpoint [IGI]
- mitotic nuclear division [IMP]
- negative regulation of JNK cascade [IMP]
- negative regulation of apoptotic process [IGI, IMP]
- negative regulation of insulin receptor signaling pathway [IGI]
- negative regulation of synaptic growth at neuromuscular junction [IMP]
- neurogenesis [IMP]
- neuron remodeling [IMP]
- positive regulation of mitotic cell cycle [IMP]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISS]
- regulation of centrosome duplication [IMP]
- regulation of protein stability [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SKP2
Gene Ontology Biological Process
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [ISM]
- compound eye development [IMP]
- endomitotic cell cycle [IMP]
- mitotic cell cycle [IMP]
- positive regulation of cell cycle [IMP]
- regulation of protein stability [IMP]
- ubiquitin-dependent protein catabolic process [ISS]
- wing disc development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A protein interaction map of Drosophila melanogaster.
Drosophila melanogaster is a proven model system for many aspects of human biology. Here we present a two-hybrid-based protein-interaction map of the fly proteome. A total of 10,623 predicted transcripts were isolated and screened against standard and normalized complementary DNA libraries to produce a draft map of 7048 proteins and 20,405 interactions. A computational method of rating two-hybrid interaction confidence ... [more]
Throughput
- High Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
SKP2 SKPA | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
SKPA SKP2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
SKP2 SKPA | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
SKPA SKP2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID