SKPA
Gene Ontology Biological Process
- DNA endoreduplication [IMP]
- centrosome duplication [IMP]
- chromosome condensation [IMP]
- mitotic G2 DNA damage checkpoint [IGI]
- mitotic nuclear division [IMP]
- negative regulation of JNK cascade [IMP]
- negative regulation of apoptotic process [IGI, IMP]
- negative regulation of insulin receptor signaling pathway [IGI]
- negative regulation of synaptic growth at neuromuscular junction [IMP]
- neurogenesis [IMP]
- neuron remodeling [IMP]
- positive regulation of mitotic cell cycle [IMP]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISS]
- regulation of centrosome duplication [IMP]
- regulation of protein stability [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SKP2
Gene Ontology Biological Process
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [ISM]
- compound eye development [IMP]
- endomitotic cell cycle [IMP]
- mitotic cell cycle [IMP]
- positive regulation of cell cycle [IMP]
- regulation of protein stability [IMP]
- ubiquitin-dependent protein catabolic process [ISS]
- wing disc development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A Drosophila protein-interaction map centered on cell-cycle regulators.
BACKGROUND: Maps depicting binary interactions between proteins can be powerful starting points for understanding biological systems. A proven technology for generating such maps is high-throughput yeast two-hybrid screening. In the most extensive screen to date, a Gal4-based two-hybrid system was used recently to detect over 20,000 interactions among Drosophila proteins. Although these data are a valuable resource for insights into ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SKP2 SKPA | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SKPA SKP2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SKP2 SKPA | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SKPA SKP2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID