SKP2
Gene Ontology Biological Process
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [ISM]
- compound eye development [IMP]
- endomitotic cell cycle [IMP]
- mitotic cell cycle [IMP]
- positive regulation of cell cycle [IMP]
- regulation of protein stability [IMP]
- ubiquitin-dependent protein catabolic process [ISS]
- wing disc development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SKPA
Gene Ontology Biological Process
- DNA endoreduplication [IMP]
- centrosome duplication [IMP]
- chromosome condensation [IMP]
- mitotic G2 DNA damage checkpoint [IGI]
- mitotic nuclear division [IMP]
- negative regulation of JNK cascade [IMP]
- negative regulation of apoptotic process [IGI, IMP]
- negative regulation of insulin receptor signaling pathway [IGI]
- negative regulation of synaptic growth at neuromuscular junction [IMP]
- neurogenesis [IMP]
- neuron remodeling [IMP]
- positive regulation of mitotic cell cycle [IMP]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [ISS]
- regulation of centrosome duplication [IMP]
- regulation of protein stability [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Cks85A and Skp2 interact to maintain diploidy and promote growth in Drosophila.
The Cks or Suc1 proteins are highly conserved small proteins that play remarkably diverse roles in the cell cycle. All Cks homologues have the ability to associate with Cyclin dependent kinases (Cdks) and in many cases this interaction has been shown to be important for function. Here we characterize the null and RNAi knockdown phenotype of the Drosophila Cks1 (Cks85A) ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SKP2 SKPA | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SKPA SKP2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SKPA SKP2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| SKPA SKP2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID