TJP2
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
KIF14
Gene Ontology Biological Process
- ATP catabolic process [IBA, ISS]
- SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IMP]
- activation of protein kinase activity [IMP]
- cell proliferation in forebrain [ISS]
- cerebellar Purkinje cell layer structural organization [ISS]
- cerebellar cortex development [ISS]
- cerebellar granular layer structural organization [ISS]
- cerebral cortex development [ISS]
- cytoskeleton-dependent intracellular transport [IBA]
- establishment of protein localization [IDA]
- hippocampus development [ISS]
- microtubule depolymerization [ISS]
- microtubule-based movement [IBA]
- mitotic cell cycle process [IMP]
- mitotic metaphase plate congression [IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of integrin activation [IMP]
- negative regulation of neuron apoptotic process [ISS]
- olfactory bulb development [ISS]
- positive regulation of cell proliferation [IDA, IMP]
- positive regulation of cytokinesis [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- regulation of G1/S transition of mitotic cell cycle [IMP]
- regulation of G2/M transition of mitotic cell cycle [IMP]
- regulation of Rap protein signal transduction [IMP]
- regulation of cell adhesion [IMP]
- regulation of cell growth [IMP]
- regulation of cell migration [IMP]
- regulation of myelination [ISS]
- regulation of neuron apoptotic process [ISS]
- response to docetaxel trihydrate [IMP]
- substrate adhesion-dependent cell spreading [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
OpenCell: Endogenous tagging for the cartography of human cellular organization.
Elucidating the wiring diagram of the human cell is a central goal of the postgenomic era. We combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. Our approach provides a data-driven description of the molecular and spatial networks that organize the proteome. Unsupervised clustering of these networks delineates ... [more]
Throughput
- High Throughput
Additional Notes
- Bait generated from library of CRISPR-edited human embryonic kidney (HEK) 293T cell lines harboring fluorescent tags on individual proteins
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
KIF14 TJP2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
TJP2 KIF14 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 1 | BioGRID | - | |
TJP2 KIF14 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 116.44 | BioGRID | 3009453 |
Curated By
- BioGRID