Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Cyclin B1/Cdk1 binds and phosphorylates Filamin A and regulates its ability to cross-link actin.

Cukier IH, Li Y, Lee JM

Substantial actin remodelling occurs prior to mitosis as cells alter their shape in preparation for cytokinesis. In mammalian cells, mitosis is initiated by a heterodimer of cyclin B1 and the cyclin dependent kinase 1 (Cdk1) serine/threonine kinase. In this report. we show that human cyclin B1 binds the actin cross-linking protein Filamin-A (FLNa). The proteins co-immunoprecipitate and co-localize in mitotic ... [more]

FEBS Lett Apr. 17, 2007; 581(8);1661-72 [Pubmed: 17408621]

Throughput

  • Low Throughput

Curated By

  • BioGRID