BAIT

GCN2

AAS1, AAS102, NDR2, serine/threonine-protein kinase GCN2, L000000681, YDR283C
Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

TIF5

SUI5, translation initiation factor eIF5, L000002305, YPR041W
Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2
Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Global phosphoproteomics pinpoints uncharted Gcn2-mediated mechanisms of translational control.

Dokladal L, Stumpe M, Pillet B, Hu Z, Garcia Osuna GM, Kressler D, Dengjel J, De Virgilio C

The conserved Gcn2 protein kinase mediates cellular adaptations to amino acid limitation through translational control of gene expression that is exclusively executed by phosphorylation of the ?-subunit of the eukaryotic translation initiation factor 2 (eIF2?). Using quantitative phosphoproteomics, however, we discovered that Gcn2 targets auxiliary effectors to modulate translation. Accordingly, Gcn2 also phosphorylates the ?-subunit of the trimeric eIF2 G protein ... [more]

Mol Cell Dec. 06, 2020; 81(9);1879-1889.e6 [Pubmed: 33743194]

Throughput

  • Low Throughput

Ontology Terms

  • vegetative growth (APO:0000106)

Additional Notes

  • mutation of Ser80 to Ala in Sui3, but not Ser80 to Glu, suppressed the growth inhibition imposed by the expression of Gcn2F842L to a similar extent as a mutation of Ser52 to Ala in Sui2 or expression of a Tif5-W391F allele with reduced affinity for Sui3

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GCN2 TIF5
Dosage Rescue
Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Low-BioGRID
522250

Curated By

  • BioGRID