BAIT

MAPK3

ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK
mitogen-activated protein kinase 3
GO Process (52)
GO Function (3)
GO Component (12)

Gene Ontology Biological Process

Homo sapiens
PREY

PI4KB

NPIK, PI4K-BETA, PI4K92, PI4KBETA, PI4KIIIBETA, PIK4CB, RP11-126K1.4
phosphatidylinositol 4-kinase, catalytic, beta
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Mitotic Golgi translocation of ERK1c is mediated by a PI4KIII?-14-3-3? shuttling complex.

Wortzel I, Hanoch T, Porat Z, Hausser A, Seger R

Golgi fragmentation is a highly regulated process that allows division of the Golgi complex between the two daughter cells. The mitotic reorganization of the Golgi is accompanied by a temporary block in Golgi functioning, as protein transport in and out of the Golgi stops. Our group has previously demonstrated the involvement of the alternatively spliced variants ERK1c and MEK1b (ERK1 ... [more]

J Cell Sci Nov. 15, 2015; 128(22);4083-95 [Pubmed: 26459638]

Throughput

  • Low Throughput

Curated By

  • BioGRID