An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Kinase Interaction Network Expands Functional and Disease Roles of Human Kinases.

Buljan M, Ciuffa R, van Drogen A, Vichalkovski A, Mehnert M, Rosenberger G, Lee S, Varjosalo M, Pernas LE, Spegg V, Snijder B, Aebersold R, Gstaiger M

Protein kinases are essential for signal transduction and control of most cellular processes, including metabolism, membrane transport, motility, and cell cycle. Despite the critical role of kinases in cells and their strong association with diseases, good coverage of their interactions is available for only a fraction of the 535 human kinases. Here, we present a comprehensive mass-spectrometry-based analysis of a ... [more]

Mol Cell Dec. 06, 2019; 79(3);504-520.e9 [Pubmed: 32707033]

Quantitative Score

31.18285714 [Protein Abundance Ratio]

Throughput

High Throughput

Additional Notes

Affinity Capture-MS was carried out to identify high confidence protein-protein interactors with a FDR<1% (protein abundance ratio compared to control are reported)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NEK4 HSP90AA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Basei FL (2015)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

NEK4

Gene Ontology Biological Process

Gene Ontology Molecular Function

manganese ion binding [ISS]protein serine/threonine kinase activity [ISS]receptor signaling protein serine/threonine kinase activity [IBA]

Gene Ontology Cellular Component

HSP90AA1