An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Kinase Interaction Network Expands Functional and Disease Roles of Human Kinases.

Buljan M, Ciuffa R, van Drogen A, Vichalkovski A, Mehnert M, Rosenberger G, Lee S, Varjosalo M, Pernas LE, Spegg V, Snijder B, Aebersold R, Gstaiger M

Protein kinases are essential for signal transduction and control of most cellular processes, including metabolism, membrane transport, motility, and cell cycle. Despite the critical role of kinases in cells and their strong association with diseases, good coverage of their interactions is available for only a fraction of the 535 human kinases. Here, we present a comprehensive mass-spectrometry-based analysis of a ... [more]

Mol Cell Dec. 06, 2019; 79(3);504-520.e9 [Pubmed: 32707033]

Quantitative Score

68.0 [Protein Abundance Ratio]

Throughput

High Throughput

Additional Notes

Affinity Capture-MS was carried out to identify high confidence protein-protein interactors with a FDR<1% (protein abundance ratio compared to control are reported)

Curated By

BioGRID

Interaction Summary

MELK

Gene Ontology Biological Process

Gene Ontology Molecular Function

calcium ion binding [IDA]non-membrane spanning protein tyrosine kinase activity [IDA]protein binding [IPI]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

LRP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

apolipoprotein binding [IDA, IPI]calcium ion binding [ISS]lipoprotein particle receptor binding [IC]lipoprotein transporter activity [NAS]poly(A) RNA binding [IDA]protein binding [IPI]protein complex binding [IDA]receptor activity [TAS]