BAIT

ORF1AB

ORF1ab polyprotein, 1ab, SARS-CoV2 ORF1ab, SARS-CoV-2 ORF1ab, Replicase, R1AB_SARS2, PRO_0000449618, GU280_gp01
Replicase polyprotein 1ab
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

N

nuc, nucleocapsid, SARS-CoV2 N, N protein, ncap, SARS-CoV-2 N, Nucleoprotein, NCAP_SARS2, PRO_0000449656, GU280_gp10
Nucleocapsid phosphoprotein; Nucleoprotein
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

The SARS-CoV-2 RNA interactome.

Lee S, Lee YS, Choi Y, Son A, Park Y, Lee KM, Kim J, Kim JS, Kim VN

SARS-CoV-2 is an RNA virus whose success as a pathogen relies on its abilities to repurpose host RNA-binding proteins (RBPs) and to evade antiviral RBPs. To uncover the SARS-CoV-2 RNA interactome, we here develop a robust ribonucleoprotein (RNP) capture protocol and identify 109 host factors that directly bind to SARS-CoV-2 RNAs. Applying RNP capture on another coronavirus, HCoV-OC43, revealed evolutionarily ... [more]

Mol Cell Jul. 01, 2021; 81(13);2838-2850.e6 [Pubmed: 33989516]

Throughput

  • High Throughput|Low Throughput

Additional Notes

  • HTP Bait RNA captured by antisense probe; High confidence protein Hit identified by MS with a FDR<10%
  • LTP Bait RNA captured by antisense probe; Hit identified by western
  • SARS-CoV-2 Wuhan-Hu-1 bait RNA and hit protein

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
N ORF1AB
Affinity Capture-RNA
Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

High-BioGRID
3558534
N ORF1AB
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
3496600
ORF1AB N
Protein-RNA
Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

High-BioGRID
-
ORF1AB N
Protein-RNA
Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

High-BioGRID
3741454

Curated By

  • BioGRID