BAIT

N

nuc, nucleocapsid, SARS-CoV2 N, N protein, ncap, SARS-CoV-2 N, Nucleoprotein, NCAP_SARS2, PRO_0000449656, GU280_gp10
Nucleocapsid phosphoprotein; Nucleoprotein
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

ORF1AB

ORF1ab polyprotein, 1ab, SARS-CoV2 ORF1ab, SARS-CoV-2 ORF1ab, Replicase, R1AB_SARS2, PRO_0000449618, GU280_gp01
Replicase polyprotein 1ab
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Defining distinct RNA-protein interactomes of SARS-CoV-2 genomic and subgenomic RNAs

Whitworth IT, Knoener R, Puray Chavez M, Halfmann P, Romero S, Baddouh M, Scalf M, Kawaoka Y, Kutluay SB, Smith LM, Sherer NM

Host RNA binding proteins recognize viral RNA and play key roles in virus replication and antiviral defense mechanisms. SARS-CoV-2 generates a series of tiered subgenomic RNAs (sgRNAs), each encoding distinct viral protein(s) that regulate different aspects of viral replication. Here, for the first time, we demonstrate the successful isolation of SARS-CoV-2 genomic RNA and three distinct sgRNAs (N, S, and ... [more]

Status: Preliminary Report

Throughput

  • High Throughput

Additional Notes

  • 24 hours post infection (24-hpi) with SARS-CoV-2
  • SARS-CoV-2 RNA and protein interaction in Huh7+AT cells modified to express the SARS-CoV-2 receptor ACE2 and entry co-factor TMPRSS2.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ORF1AB N
Affinity Capture-RNA
Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Low/High-BioGRID
3490080
N ORF1AB
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
3496600
ORF1AB N
Protein-RNA
Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

High-BioGRID
-
ORF1AB N
Protein-RNA
Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

High-BioGRID
3741454

Curated By

  • BioGRID