BAIT

N

nuc, nucleocapsid, SARS-CoV2 N, N protein, ncap, SARS-CoV-2 N, Nucleoprotein, NCAP_SARS2, PRO_0000449656, GU280_gp10
Nucleocapsid phosphoprotein; Nucleoprotein
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

ORF1AB

ORF1ab polyprotein, 1ab, SARS-CoV2 ORF1ab, SARS-CoV-2 ORF1ab, Replicase, R1AB_SARS2, PRO_0000449618, GU280_gp01
Replicase polyprotein 1ab
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

The intraviral protein-protein interaction of SARS-CoV-2 reveals the key role of N protein in virus-like particle assembly.

Chen M, Yan C, Qin F, Zheng L, Zhang XE

Intraviral protein-protein interactions (PPIs) of SARS-CoV-2 in host cells may provide useful information for deep understanding of virology of SARS-CoV-2. In this study, 22 of 55 interactions of the structural and accessory proteins of SARS-CoV-2 were identified by biomolecular fluorescence complementation (BiFC) assay. The nucleocapsid (N) protein was found to have the most interactions among the structural and accessory proteins ... [more]

Int J Biol Sci Oct. 22, 2021; 17(14);3889-3897 [Pubmed: 34671206]

Throughput

  • Low Throughput

Additional Notes

  • iRFP-based TriFC assay for protein-RNA interaction; Hit is PS576

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ORF1AB N
Affinity Capture-RNA
Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Low/High-BioGRID
3490080
N ORF1AB
Affinity Capture-RNA
Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

High-BioGRID
3558534
ORF1AB N
Protein-RNA
Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

High-BioGRID
-
ORF1AB N
Protein-RNA
Protein-RNA

An interaction is detected between and protein and an RNA in vitro.

High-BioGRID
3741454

Curated By

  • BioGRID