BAIT

CSNK2A2

CK2A2, CSNK2A1
casein kinase 2, alpha prime polypeptide
GO Process (3)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

EFCAB14

KIAA0494, RP11-8J9.3
EF-hand calcium binding domain 14
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Multiplexed kinase interactome profiling quantifies cellular network activity and plasticity.

Golkowski M, Lius A, Sapre T, Lau HT, Moreno T, Maly DJ, Ong SE

Dynamic changes in protein-protein interaction (PPI) networks underlie all physiological cellular functions and drive devastating human diseases. Profiling PPI networks can, therefore, provide critical insight into disease mechanisms and identify new drug targets. Kinases are regulatory nodes in many PPI networks; yet, facile methods to systematically study kinase interactome dynamics are lacking. We describe kinobead competition and correlation analysis (kiCCA), ... [more]

Mol Cell Mar. 02, 2023; 83(5);803-818.e8 [Pubmed: 36736316]

Quantitative Score

  • 0.944779327 [kiCCA Pearson R Value]

Throughput

  • High Throughput

Additional Notes

  • A kinobead competition and correlation analysis (kiCCA) involving a quantitative mass spectrometry-based chemoproteomic method was carried out to identify endogenous kinase interactors.
  • High confidence interactions had a kiCCA Pearson R Value >=0.6.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CSNK2A2 EFCAB14
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High884BioGRID
3481525
CSNK2A2 EFCAB14
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
3353092
CSNK2A2 EFCAB14
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
941085

Curated By

  • BioGRID