GPB1
Gene Ontology Biological Process
- glucose mediated signaling pathway [IGI, IMP]
- invasive growth in response to glucose limitation [IGI, IMP]
- negative regulation of Ras protein signal transduction [IGI]
- negative regulation of phosphorylation [IGI]
- positive regulation of Ras protein signal transduction [IMP]
- protein polyubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA, IMP]
- pseudohyphal growth [IGI, IMP]
- signal transduction [IPI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GPB2
Gene Ontology Biological Process
- ascospore formation [IMP]
- glucose mediated signaling pathway [IGI, IMP]
- invasive growth in response to glucose limitation [IGI, IMP]
- negative regulation of Ras protein signal transduction [IGI]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- pseudohyphal growth [IGI, IMP]
- signal transduction [IPI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The social and structural architecture of the yeast protein interactome.
Cellular functions are mediated by protein-protein interactions, and mapping the interactome provides fundamental insights into biological systems. Affinity purification coupled to mass spectrometry is an ideal tool for such mapping, but it has been difficult to identify low copy number complexes, membrane complexes and complexes that are disrupted by protein tagging. As a result, our current knowledge of the interactome ... [more]
Quantitative Score
- 2.0 [Score_FDR+correlation]
Throughput
- High Throughput
Additional Notes
- Protein interactions were identified using statistically significant enrichment of the proteins in the forward and reverse pull-downs, as well as making use of the profile similarities of interacting proteins in a correlation analysis. High confidence interactions have a total score >=2. This score is a sum of the FDR score of the forward pull-down + FDR score of the reverse pull-down + correlation score.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GPB2 GPB1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.194 | BioGRID | 354456 | |
| GPB2 GPB1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.154 | BioGRID | 2076443 | |
| GPB2 GPB1 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 156334 | |
| GPB1 GPB2 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 156266 |
Curated By
- BioGRID