BAIT

SYK

p72-Syk
spleen tyrosine kinase
GO Process (38)
GO Function (6)
GO Component (8)

Gene Ontology Biological Process

Homo sapiens
PREY

NEDD4L

NEDD4-2, NEDD4.2, RSP5, hNEDD4-2
neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase
GO Process (33)
GO Function (7)
GO Component (7)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Bacterial DNA promoting inflammation via the Sgk1/Nedd4L/Syk pathway in mast cells contributes to antihistamine-nonresponsive CSU.

Chen B, Song Y, Yang X, Yang J, Hao F

Inflammation centered on non-IgE-mediated mast cell activation characterizes chronic spontaneous urticaria resistant to nonsedating H1-antihistamines. We recently uncovered a strong positive association between inflammation and the fecal Escherichia. To further explore the actions of bacterial DNA derived from Escherichia on mast cells, intestinal permeability of patients with chronic spontaneous urticaria with or without nonsedating H1-antihistamine resistance and healthy controls were ... [more]

J Leukoc Biol May. 02, 2023; 113(5);461-470 [Pubmed: 36857592]

Throughput

  • Low Throughput

Curated By

  • BioGRID