CPA
Gene Ontology Biological Process
- actin cytoskeleton organization [IMP, NAS]
- actin filament organization [IMP]
- barbed-end actin filament capping [IMP, ISS, NAS]
- cell morphogenesis [IMP]
- germarium-derived oocyte fate determination [IMP]
- microtubule-based movement [ISS]
- negative regulation of JNK cascade [IGI]
- negative regulation of actin filament polymerization [IMP]
- negative regulation of filopodium assembly [IMP]
- phagocytosis [IMP]
- regulation of filopodium assembly [IMP]
- regulation of lamellipodium assembly [IMP]
- wing disc development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CPB
Gene Ontology Biological Process
- actin cytoskeleton organization [IMP]
- actin filament organization [IMP]
- barbed-end actin filament capping [IDA, IMP]
- border follicle cell migration [IMP]
- cell morphogenesis [IMP]
- cytoplasmic transport, nurse cell to oocyte [IMP]
- negative regulation of JNK cascade [IGI]
- negative regulation of filopodium assembly [IMP]
- positive regulation of synaptic growth at neuromuscular junction [IMP]
- regulation of actin filament length [IMP]
- regulation of lamellipodium assembly [IMP]
- wing disc development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Next-generation large-scale binary protein interaction network for Drosophila melanogaster.
Generating reference maps of interactome networks illuminates genetic studies by providing a protein-centric approach to finding new components of existing pathways, complexes, and processes. We apply state-of-the-art methods to identify binary protein-protein interactions (PPIs) for Drosophila melanogaster. Four all-by-all yeast two-hybrid (Y2H) screens of?>?10,000 Drosophila proteins result in the 'FlyBi' dataset of 8723 PPIs among 2939 proteins. Testing subsets of ... [more]
Throughput
- High Throughput
Additional Notes
- MAmmalian Protein-Protein Interaction Trap (MAPPIT) assay
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CPB CPA | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| CPA CPB | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - | |
| CPB CPA | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 3712649 |
Curated By
- BioGRID