An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The ubiquitin- and proteasome-dependent degradation of COX-2 is regulated by the COP9 signalosome and differentially influenced by coxibs.

Neuss H, Huang X, Hetfeld BK, Deva R, Henklein P, Nigam S, Mall JW, Schwenk W, Dubiel W

The cyclooxygenase-2 (COX-2) enzyme is induced upon inflammation and in neoplastic tissues. It produces prostaglandins that stimulate tumor angiogenesis and tumor growth. Therefore, destruction and/or specific inhibition of COX-2 should be an important aspect of future tumor therapy. Recently, clinical application of specific COX-2 inhibitors called coxibs became doubtfully because they produce serious renal and cardiovascular complications under long term ... [more]

J. Mol. Med. Sep. 01, 2007; 85(9);961-70 [Pubmed: 17429597]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PTGS2

Gene Ontology Biological Process

Gene Ontology Molecular Function

arachidonate 15-lipoxygenase activity [TAS]enzyme binding [IPI]heme binding [ISS]peroxidase activity [NAS]prostaglandin-endoperoxide synthase activity [IDA]

Gene Ontology Cellular Component

COPS3