ESC4, L000004424, YHR154W
Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
GO Process (2)
GO Function (0)
GO Component (2)
Saccharomyces cerevisiae (S288c)


E3 ubiquitin-protein ligase BRE1, YDL074C
E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control; interacts with RNA-binding protein Npl3p, linking histone ubiquitination to mRNA processing; Bre1p-dependent histone ubiquitination promotes pre-mRNA splicing
Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.


Loss of H3 K79 trimethylation leads to suppression of RTT107-dependent DNA damage sensitivity through the translesion synthesis pathway.

Levesque N, Leung GP, Fok AK, Schmidt TI, Kobor MS

Genomic integrity is maintained by the coordinated interaction of many DNA damage response pathways, including checkpoints, DNA repair processes, and cell cycle restart. In Saccharomyces cerevisiae,, the BRCT domain-containing protein Rtt107/Esc4 is required for restart of DNA replication after successful repair of DNA damage, and for cellular resistance to DNA damaging agents. Rtt107 and its interaction partner Slx4 are phosphorylated ... [more]

Unknown Sep. 01, 2010; 0(0); [Pubmed: 20810656]


  • Low Throughput

Ontology Terms

  • phenotype: viability (APO:0000111)
  • phenotype: resistance to chemicals (APO:0000087)

Additional Notes

  • double mutants show decreased sensitivity to MMS (PubCHEM ID: 4156 CHEBI ID: 25255)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.


Curated By

  • BioGRID