BAIT

LET-60

CELE_ZK792.6, lin-34, ZK792.6
let-60 encodes a member of the GTP-binding RAS protooncogene family; let-60 activity is required for viability, vulval development, spicule development, germ line meiotic progression, posterior development of the hypodermis, chemotaxis, sex myoblast migration, and muscle membrane extension; let-60 acts genetically downstream of let-23 with respect to vulval development and upstream of the MAPK pathway with respect to chemotaxis; let-60 is expressed in neural, muscle, and hypodermal lineages.
Caenorhabditis elegans
PREY

DSH-2

CELE_C27A2.6, C27A2.6
dsh-2 encodes one of three C. elegans Dishevelled (Dsh) homologs; during embryonic development, dsh-2 functions in the Wnt signaling pathway that specifies endoderm and orients the division axis of the EMS blastomere; dsh-2 is also required for many neuronal cell fate specifications and for asymmetric cell division in the early somatic gonad; dsh-2 is additionally required for proper hypodermal morphogenesis during embryonic elongation; DSH-2 is expressed in the embryo from the early stages of embryogenesis through the ~1.5-fold stage; DSH-2 is also expressed in the somatic gonad precursors Z1 and Z4; DSH-2 localizes to the cell cortex.
Caenorhabditis elegans

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Systematic mapping of genetic interactions in Caenorhabditis elegans identifies common modifiers of diverse signaling pathways.

Lehner B, Crombie C, Tischler J, Fortunato A, Fraser AG

Most heritable traits, including disease susceptibility, are affected by interactions between multiple genes. However, we understand little about how genes interact because very few possible genetic interactions have been explored experimentally. We have used RNA interference in Caenorhabditis elegans to systematically test approximately 65,000 pairs of genes for their ability to interact genetically. We identify approximately 350 genetic interactions between ... [more]

Nat. Genet. Aug. 01, 2006; 38(8);896-903 [Pubmed: 16845399]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: embryonic lethal (WBPHENOTYPE:0000050)
  • phenotype: sterile (WBPHENOTYPE:0000688)

Additional Notes

  • A large-scale genetic interaction analysis was carried out to detect interactions between 37 query mutants and approximately 1,750 target genes compromised by RNA interference (RNAi). Interactions were identified if the phenotype of the combined query-target perturbation was greater than the sum of the two individual perturbations.
  • Synthetic

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LET-60 DSH-2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High1.8BioGRID
466129

Curated By

  • BioGRID