BAIT

SIT4

PPH1, type 2A-related serine/threonine-protein phosphatase SIT4, L000001901, YDL047W
Type 2A-related serine-threonine phosphatase; functions in the G1/S transition of the mitotic cycle; regulator of COPII coat dephosphorylation; required for ER to Golgi traffic; interacts with Hrr25p kinase; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization; similar to human PP6
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

PPH21

phosphoprotein phosphatase 2A catalytic subunit PPH21, L000001472, YDL134C
Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Intra-Nuclear Function for PP2A Phosphatase: Pph21/22 Are Required for Rapamycin-Induced GATA Factor Binding to the DAL5 Promoter in Yeast.

Georis I, Tate JJ, Feller A, Cooper TG, Dubois E

PP2A, a central Tor pathway phosphatase consisting of a catalytic subunit (Pph21 or Pph22), a scaffold subunit (Tpd3) and one of two regulatory subunits (Cdc55 or Rts1) has been repeatedly shown to play important roles in cytoplasmically-localized signal transduction activities. In contrast, its involvement in intra-nuclear control of messenger RNA production has heretofore not been reported. Here, we demonstrate for ... [more]

Unknown Oct. 25, 2010; 0(0); [Pubmed: 20974806]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: rna accumulation (APO:0000224)

Additional Notes

  • genetic complex
  • triple mutants show a modest decrease in DAL5 mRNA levels

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SIT4 PPH21
Dosage Rescue
Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Low-BioGRID
520082
SIT4 PPH21
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
435579
SIT4 PPH21
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
1238183
PPH21 SIT4
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
1238185

Curated By

  • BioGRID