BAIT
TOPBP1
TOP2BP1
topoisomerase (DNA) II binding protein 1
GO Process (4)
GO Function (3)
GO Component (7)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TOP2B
TOPIIB, top2beta
topoisomerase (DNA) II beta 180kDa
GO Process (7)
GO Function (7)
GO Component (8)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
A DNA-topoisomerase-II-binding protein with eight repeating regions similar to DNA-repair enzymes and to a cell-cycle regulator.
A two-hybrid system was used to isolate factors that interact with the C-terminal region of DNA topoisomerase IIbeta. A positive clone isolated from a HeLa cDNA library encoded 1522 amino acid residues (molecular mass 170670). The protein, designated topoisomerase-IIbeta-binding protein 1 (TopBP1), interacted with the C-terminal region of topoisomerase IIbeta synthesized in vitro. A database search indicated that TopBP1 possessed ... [more]
Eur. J. Biochem. Dec. 15, 1997; 250(3);794-9 [Pubmed: 9461304]
Throughput
- Low Throughput
Curated By
- BioGRID