PAF1
Gene Ontology Biological Process
- DNA-templated transcription, termination [IMP]
- chromatin organization involved in regulation of transcription [IMP]
- chromatin silencing at rDNA [IMP]
- global genome nucleotide-excision repair [IMP]
- mRNA 3'-end processing [IMP]
- negative regulation of DNA recombination [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of histone H3-K36 trimethylation [IMP]
- positive regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]
- positive regulation of transcription elongation from RNA polymerase I promoter [IDA]
- positive regulation of transcription elongation from RNA polymerase II promoter [IMP]
- rRNA processing [IMP]
- regulation of chromatin silencing at telomere [IMP]
- regulation of histone H2B conserved C-terminal lysine ubiquitination [IDA]
- regulation of histone H2B ubiquitination [IMP]
- regulation of histone H3-K4 methylation [IMP]
- regulation of phosphorylation of RNA polymerase II C-terminal domain serine 2 residues [IMP]
- regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of transcription involved in G1/S transition of mitotic cell cycle [IMP]
- regulation of transcription-coupled nucleotide-excision repair [IGI]
- snoRNA 3'-end processing [IMP]
- snoRNA transcription from an RNA polymerase II promoter [IDA, IMP]
- transcription elongation from RNA polymerase I promoter [IMP]
- transcription elongation from RNA polymerase II promoter [IGI, IMP]
- transcription from RNA polymerase I promoter [IGI, IMP]
Gene Ontology Molecular Function- RNA polymerase II C-terminal domain phosphoserine binding [IDA]
- RNA polymerase II core binding [IPI]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IPI]
- TFIIF-class binding transcription factor activity [IMP, IPI]
- chromatin binding [IDA]
- RNA polymerase II C-terminal domain phosphoserine binding [IDA]
- RNA polymerase II core binding [IPI]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity [IPI]
- TFIIF-class binding transcription factor activity [IMP, IPI]
- chromatin binding [IDA]
Gene Ontology Cellular Component
RPB3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Mpk1 MAPK association with the paf1 complex blocks sen1-mediated premature transcription termination.
The Mpk1 MAPK of the yeast cell wall integrity pathway uses a noncatalytic mechanism to activate transcription of stress-induced genes by recruitment of initiation factors to target promoters. We show here that Mpk1 additionally serves a function in transcription elongation that is also independent of its catalytic activity. This function is mediated by an interaction between Mpk1 and the Paf1 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RPB3 PAF1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3309310 | |
| RPB3 PAF1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 2200120 | |
| PAF1 RPB3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 5 | BioGRID | 3614465 | |
| PAF1 RPB3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RPB3 PAF1 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID