BAIT

GRR1

CAT80, COT2, SDC1, SSU2, SCF ubiquitin ligase complex subunit GRR1, L000000730, YJR090C

F-box protein component of an SCF ubiquitin-ligase complex; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCF(Grr1) complex; SCF(Grr1) acts as a ubiquitin-protein ligase directing ubiquitination of substrates such as: Gic2p, Mks1p, Mth1p, Cln1p, Cln2p and Cln3p; involved in carbon catabolite repression, glucose-dependent divalent cation transport, glucose transport, morphogenesis, and sulfite detoxification

Kinome Project (Sc)

UPS Project

GO Process (7)

GO Function (2)

GO Component (4)

Gene Ontology Biological Process

G1/S transition of mitotic cell cycle [TAS]

SCF-dependent proteasomal ubiquitin-dependent protein catabolic process [IPI]

cellular response to DNA damage stimulus [IMP]

cellular response to methylmercury [IMP]

mitotic cell cycle arrest in response to pheromone [IMP]

protein polyubiquitination [IMP]

protein ubiquitination [IGI, IPI]

Gene Ontology Molecular Function

protein binding, bridging [IDA, IMP]
ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

SCF ubiquitin ligase complex [IMP]

cellular bud neck contractile ring [IDA]

cytoplasm [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

STP1

BAP1, SSY2, L000002132, YDR463W

Transcription factor; contains a N-terminal regulatory motif (RI) that acts as a cytoplasmic retention determinant and as an Asi dependent degron in the nucleus; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication

GO Process (2)

GO Function (2)

GO Component (3)

Gene Ontology Biological Process

positive regulation of transcription from RNA polymerase II promoter [IGI, IMP, ISS]

positive regulation of transcription, DNA-templated [IDA]

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleus [IDA]

plasma membrane [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Differential regulation of transcription factors Stp1 and Stp2 in the Ssy1-Ptr3-Ssy5 amino acid sensing pathway.

Tumusiime S, Zhang C, Overstreet MS, Liu Z

Stp1 and Stp2 are two homologous transcription factors activated in response to extracellular amino acid stimuli. Here we show that both ubiquitin-dependent degradation of Stp1 and Stp2 and their intracellular localization are differentially regulated. We have found that the E2 ubiquitin-conjugating enzyme Cdc34 is required for degradation of both full-length and processed Stp1, but not Stp2. We have also found ... [more]

J. Biol. Chem. Feb. 11, 2011; 286(6);4620-31 [Pubmed: 21127045]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

GRR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding, bridging [IDA, IMP]ubiquitin-protein transferase activity [IDA]

Gene Ontology Cellular Component

STP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]

Gene Ontology Cellular Component

Affinity Capture-Western

Publication

Differential regulation of transcription factors Stp1 and Stp2 in the Ssy1-Ptr3-Ssy5 amino acid sensing pathway.

Throughput

Curated By

protein binding, bridging [IDA, IMP]
ubiquitin-protein transferase activity [IDA]

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]