BAIT
APC
AI047805, AU020952, AW124434, CC1, Min, mAPC
adenomatosis polyposis coli
GO Process (59)
GO Function (9)
GO Component (30)
Gene Ontology Biological Process
- T cell differentiation in thymus [IMP]
- Wnt signaling pathway [IDA, IMP]
- anterior/posterior pattern specification [IMP]
- axis specification [IMP]
- axonogenesis [IGI, IMP]
- canonical Wnt signaling pathway [IMP]
- canonical Wnt signaling pathway involved in negative regulation of apoptotic process [IGI]
- canonical Wnt signaling pathway involved in positive regulation of apoptotic process [IGI]
- cell cycle arrest [ISO]
- cell migration [IMP, ISO]
- cellular response to DNA damage stimulus [ISO]
- chromosome organization [IGI, IMP]
- cytoplasmic microtubule organization [IGI]
- dorsal/ventral pattern formation [IMP]
- establishment or maintenance of cell polarity [ISO]
- hair follicle development [IMP]
- kidney development [IMP]
- metaphase/anaphase transition of mitotic cell cycle [IGI, IMP]
- mitotic cytokinesis [IMP, ISO]
- mitotic spindle assembly checkpoint [ISO]
- muscle cell cellular homeostasis [IGI]
- negative regulation of MAPK cascade [IGI, IMP]
- negative regulation of Wnt signaling pathway [IGI, IMP]
- negative regulation of apoptotic process [IMP]
- negative regulation of canonical Wnt signaling pathway [IMP, ISO]
- negative regulation of cell proliferation [IGI, IMP, ISO]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [ISO]
- negative regulation of epithelial cell proliferation [IGI, IMP]
- negative regulation of epithelial cell proliferation involved in prostate gland development [IMP]
- negative regulation of microtubule depolymerization [IMP, ISO]
- negative regulation of odontogenesis [IMP]
- neuron projection development [ISO]
- pattern specification process [IMP]
- positive regulation of apoptotic process [IMP, ISO]
- positive regulation of cell adhesion [IMP]
- positive regulation of cell differentiation [IMP]
- positive regulation of cell division [IMP]
- positive regulation of cell migration [IMP, ISO]
- positive regulation of epithelial cell differentiation [IGI]
- positive regulation of microtubule polymerization [IMP]
- positive regulation of protein catabolic process [IMP, ISO]
- positive regulation of pseudopodium assembly [ISO]
- protein complex assembly [ISO]
- proximal/distal pattern formation [IMP]
- regulation of attachment of spindle microtubules to kinetochore [IMP, ISO]
- regulation of cell cycle [IMP]
- regulation of cell differentiation [IGI]
- regulation of cell migration [IGI, IMP]
- regulation of epithelial cell differentiation [IMP]
- regulation of glutamate metabolic process [ISO]
- regulation of microtubule-based process [ISO]
- regulation of nitrogen compound metabolic process [IMP]
- regulation of osteoblast differentiation [IMP]
- regulation of osteoclast differentiation [IMP]
- retina development in camera-type eye [IMP]
- skin development [IMP]
- somatic stem cell maintenance [IGI, IMP]
- stem cell maintenance [IMP]
- thymus development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Scrib-APC-beta-catenin complex [IDA]
- axon [IDA]
- axonal growth cone [IDA, ISO]
- beta-catenin destruction complex [ISO]
- cell [IGI]
- cell body fiber [ISO]
- cell cortex [ISO]
- cell projection [IDA]
- cell projection membrane [IDA]
- cell-cell adherens junction [ISO]
- centrosome [ISO]
- cytoplasm [IDA, ISO]
- cytoplasmic microtubule [IDA]
- dendrite [ISO]
- growth cone [IDA, ISO]
- kinetochore [ISO]
- lamellipodium [ISO]
- lateral plasma membrane [ISO]
- microtubule [ISO]
- microtubule plus-end [IDA]
- neuron projection [ISO]
- neuron projection terminus [ISO]
- neuronal cell body [ISO]
- nuclear membrane [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO]
- protein complex [ISO]
- ruffle membrane [ISO]
- synapse [ISO]
- tight junction [ISO]
Mus musculus
PREY
AXIN2
Axi1, Axil, Conductin, RP23-202G21.3
axin2
GO Process (30)
GO Function (8)
GO Component (13)
Gene Ontology Biological Process
- Wnt signaling pathway involved in somitogenesis [IBA]
- bone mineralization [IMP]
- cell death [IBA, ISO]
- cell proliferation [IMP]
- cellular protein localization [ISO]
- cellular response to organic cyclic compound [IBA]
- chondrocyte differentiation involved in endochondral bone morphogenesis [IMP]
- dorsal/ventral axis specification [IBA]
- intramembranous ossification [IMP]
- mRNA stabilization [ISO]
- maintenance of DNA repeat elements [ISO]
- negative regulation of Wnt signaling pathway involved in dorsal/ventral axis specification [ISO]
- negative regulation of canonical Wnt signaling pathway [IBA, ISO]
- negative regulation of catenin import into nucleus [ISO]
- negative regulation of cell proliferation [IMP, ISO]
- negative regulation of osteoblast differentiation [IMP]
- odontogenesis [ISO]
- positive regulation of GTPase activity [IBA]
- positive regulation of cell death [ISO]
- positive regulation of epithelial to mesenchymal transition [ISO]
- positive regulation of protein kinase activity [ISO]
- positive regulation of protein phosphorylation [ISO]
- regulation of Wnt signaling pathway [IDA]
- regulation of catenin import into nucleus [IBA]
- regulation of centromeric sister chromatid cohesion [IMP, ISO]
- regulation of chondrocyte development [IMP]
- regulation of mismatch repair [ISO]
- regulation of transcription, DNA-templated [IBA]
- secondary heart field specification [IGI]
- somitogenesis [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [ISO]
- beta-catenin destruction complex [IBA]
- cell cortex [IBA]
- centrosome [IDA, ISO]
- cytoplasm [ISO]
- cytoplasmic membrane-bounded vesicle [IBA]
- cytoplasmic microtubule [IBA]
- microtubule cytoskeleton [ISO]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- plasma membrane [IBA]
- postsynaptic density [IBA]
- protein complex [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Functional interaction of an axin homolog, conductin, with beta-catenin, APC, and GSK3beta.
Control of stability of beta-catenin is central in the wnt signaling pathway. Here, the protein conductin was found to form a complex with both beta-catenin and the tumor suppressor gene product adenomatous polyposis coli (APC). Conductin induced beta-catenin degradation, whereas mutants of conductin that were deficient in complex formation stabilized beta-catenin. Fragments of APC that contained a conductin-binding domain also ... [more]
Science Apr. 24, 1998; 280(5363);596-9 [Pubmed: 9554852]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| APC AXIN2 | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 521260 | |
| APC AXIN2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID