BAIT

APC

AI047805, AU020952, AW124434, CC1, Min, mAPC
adenomatosis polyposis coli
GO Process (59)
GO Function (9)
GO Component (30)

Gene Ontology Biological Process

Mus musculus
PREY

AXIN2

Axi1, Axil, Conductin, RP23-202G21.3
axin2
GO Process (30)
GO Function (8)
GO Component (13)
Mus musculus

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Functional interaction of an axin homolog, conductin, with beta-catenin, APC, and GSK3beta.

Behrens J, Jerchow BA, Wuertele M, Grimm J, Asbrand C, Wirtz R, Kuehl M, Wedlich D, Birchmeier W

Control of stability of beta-catenin is central in the wnt signaling pathway. Here, the protein conductin was found to form a complex with both beta-catenin and the tumor suppressor gene product adenomatous polyposis coli (APC). Conductin induced beta-catenin degradation, whereas mutants of conductin that were deficient in complex formation stabilized beta-catenin. Fragments of APC that contained a conductin-binding domain also ... [more]

Science Apr. 24, 1998; 280(5363);596-9 [Pubmed: 9554852]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: abnormal protein level (MP:0008469)

Additional Notes

  • transfection of Axin2 into the Apc mutant background abolishes the build-up of Beta-catenin normally seen in this mutant

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
APC AXIN2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
APC AXIN2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID