BAIT

YKU70

HDF1, NES24, KU70, L000000759, YMR284W
Subunit of the telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein to nucleus via interaction with the TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
Saccharomyces cerevisiae (S288c)
PREY

CLB2

B-type cyclin CLB2, L000000350, YPR119W
B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB2 has a paralog, CLB1, that arose from the whole genome duplication
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Distinct Cdk1 Requirements during Single-Strand Annealing, Noncrossover, and Crossover Recombination.

Trovesi C, Falcettoni M, Lucchini G, Clerici M, Longhese MP

Repair of DNA double-strand breaks (DSBs) by homologous recombination (HR) in haploid cells is generally restricted to S/G2 cell cycle phases, when DNA has been replicated and a sister chromatid is available as a repair template. This cell cycle specificity depends on cyclin-dependent protein kinases (Cdk1 in Saccharomyces cerevisiae), which initiate HR by promoting 5'-3' nucleolytic degradation of the DSB ... [more]

PLoS Genet. Aug. 01, 2011; 7(8);e1002263 [Pubmed: 21901114]

Throughput

  • Low Throughput

Ontology Terms

  • chromosome/plasmid maintenance (APO:0000143)

Additional Notes

  • Overexpression of Clb2 causes the appearance of cross-over products in yku70/rad9 mutant cells
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
YKU70 CLB2
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.9831BioGRID
809039

Curated By

  • BioGRID