BAIT
CBK1
serine/threonine protein kinase CBK1, L000004609, YNL161W
Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
GO Process (6)
GO Function (1)
GO Component (9)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
RPH1
KDM4, L000004504, YER169W
JmjC domain-containing histone demethylase; specifically demethylates H3K36 tri- and dimethyl modification states; associates with actively transcribed (RNAP II) regions in vivo and specifically targets H3K36 in its trimethylation state as its substrate; transcriptional repressor of PHR1; Rph1p phosphorylation during DNA damage is under control of the MEC1-RAD53 pathway; target of stess-induced hormesis; RPH1 has a paralog, GIS1, that arose from the whole genome duplication
GO Process (2)
GO Function (4)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA, IMP]
- histone demethylase activity (H3-K36 specific) [IDA, IMP]
- histone demethylase activity (H3-K9 specific) [IDA]
- sequence-specific DNA binding [IDA, IMP]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA, IMP]
- histone demethylase activity (H3-K36 specific) [IDA, IMP]
- histone demethylase activity (H3-K9 specific) [IDA]
- sequence-specific DNA binding [IDA, IMP]
Saccharomyces cerevisiae (S288c)
Dosage Rescue
A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.
Publication
Cbk1 kinase and Bck2 control MAP kinase activation and inactivation during heat shock.
Saccharomyces cerevisiae Cbk1 kinase is a LATS/NDR tumor suppressor orthologue and component of the RAM signaling network. Cbk1 was previously implicated in regulating polarized morphogenesis, gene expression and cell integrity. Here, we establish that Cbk1 is critical for heat shock and cell wall stress signaling via Bck2, a protein associated with the Pkc1-Mpk1 cell integrity pathway. We demonstrate that cbk1 ... [more]
Unknown Oct. 26, 2011; 0(0); [Pubmed: 22031291]
Throughput
- Low Throughput
Ontology Terms
- heat sensitivity (APO:0000147)
- vegetative growth (APO:0000106)
Additional Notes
- Overexpression of Bck2 or Rph1 partially rescues the temperature sensitivity of Cbk1
Curated By
- BioGRID