An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A lentiviral functional proteomics approach identifies chromatin remodeling complexes important for the induction of pluripotency.

Mak AB, Ni Z, Hewel JA, Chen GI, Zhong G, Karamboulas K, Blakely K, Smiley S, Marcon E, Roudeva D, Li J, Olsen JB, Wan C, Punna T, Isserlin R, Chetyrkin S, Gingras AC, Emili A, Greenblatt J, Moffat J

Protein complexes and protein-protein interactions are essential for almost all cellular processes. Here, we establish a mammalian affinity purification and lentiviral expression (MAPLE) system for characterizing the subunit compositions of protein complexes. The system is flexible (i.e. multiple N- and C-terminal tags and multiple promoters), is compatible with Gateway cloning, and incorporates a reference peptide. Its major advantage is that ... [more]

Mol. Cell Proteomics May. 01, 2010; 9(5);811-23 [Pubmed: 20305087]

Throughput

Low Throughput

Ontology Terms

cell line: hek-293 cell (BTO:0000007)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CDK9 CCT8	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Jeronimo C (2007)	High	0.9894	BioGRID	424921

Curated By

BioGRID

Interaction Summary

CDK9

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]RNA polymerase II carboxy-terminal domain kinase activity [IDA]protein binding [IPI]protein kinase activity [TAS]

Gene Ontology Cellular Component

CCT8

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity, coupled [TAS]protein binding [IPI]