PAF1
Gene Ontology Biological Process
- cellular response to lipopolysaccharide [ISS]
- endodermal cell fate commitment [ISS]
- histone H2B ubiquitination [IDA]
- histone monoubiquitination [IDA]
- mRNA polyadenylation [IMP]
- negative regulation of myeloid cell differentiation [IDA]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of mRNA 3'-end processing [IMP]
- positive regulation of transcription elongation from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
WDR61
Gene Ontology Biological Process
- histone H3-K4 trimethylation [IMP]
- negative regulation of myeloid cell differentiation [IDA]
- positive regulation of histone H3-K4 methylation [IMP]
- positive regulation of histone H3-K79 methylation [IMP]
- positive regulation of transcription elongation from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A lentiviral functional proteomics approach identifies chromatin remodeling complexes important for the induction of pluripotency.
Protein complexes and protein-protein interactions are essential for almost all cellular processes. Here, we establish a mammalian affinity purification and lentiviral expression (MAPLE) system for characterizing the subunit compositions of protein complexes. The system is flexible (i.e. multiple N- and C-terminal tags and multiple promoters), is compatible with Gateway cloning, and incorporates a reference peptide. Its major advantage is that ... [more]
Throughput
- Low Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PAF1 WDR61 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3160679 | |
| WDR61 PAF1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3034753 | |
| WDR61 PAF1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 111.4225 | BioGRID | 2941701 | |
| PAF1 WDR61 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| WDR61 PAF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 669125 | |
| PAF1 WDR61 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 3452765 | |
| PAF1 WDR61 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 1 | BioGRID | 1274555 |
Curated By
- BioGRID