BAIT
ELM1
LDB9, serine/threonine protein kinase ELM1, L000000548, YKL048C
Serine/threonine protein kinase that regulates cellular morphogenesis; septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring
GO Process (11)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
- axial cellular bud site selection [TAS]
- budding cell bud growth [IMP]
- cell morphogenesis [IMP]
- cytokinesis checkpoint [TAS]
- glucose metabolic process [IGI, IMP]
- positive regulation of protein autophosphorylation [IDA, IMP]
- protein autophosphorylation [IDA, IMP]
- protein phosphorylation [IDA, IGI]
- pseudohyphal growth [IMP]
- response to drug [IMP]
- response to osmotic stress [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
DMA1
CHF1, ubiquitin-conjugating protein DMA1, S000029719, YHR115C
Ubiquitin-protein ligase (E3); controls septin dynamics, spindle position checkpoint (SPOC) with ligase Dma2p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8, similar to human Chfr; contains FHA, RING fingers; DMA1 has a paralog, DMA2, that arose from the whole genome duplication
GO Process (8)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
- cellular bud neck septin ring organization [IGI]
- establishment of mitotic spindle orientation [IGI]
- mitotic spindle orientation checkpoint [IGI]
- protein autoubiquitination [IDA]
- protein localization to bud neck [IGI]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IGI]
- septin ring assembly [IGI]
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Budding yeast dma proteins control septin dynamics and the spindle position checkpoint by promoting the recruitment of the elm1 kinase to the bud neck.
The first step towards cytokinesis in budding yeast is the assembly of a septin ring at the future site of bud emergence. Integrity of this ring is crucial for cytokinesis, proper spindle positioning, and the spindle position checkpoint (SPOC). This checkpoint delays mitotic exit and cytokinesis as long as the anaphase spindle does not properly align with the division axis. ... [more]
PLoS Genet. Apr. 01, 2012; 8(4);e1002670 [Pubmed: 22570619]
Throughput
- Low Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- genetic complex
- lack of Dma proteins caused cell lethality or severe growth defects when combined with conditional septin mutations or with GIN4, HSL1 and ELM1 deletion
Related interactions
Curated By
- BioGRID