BAIT

SLG1

HCS77, WSC1, L000003080, YOR008C
Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
Saccharomyces cerevisiae (S288c)
PREY

BCK1

LAS3, SAP3, SLK1, SSP31, mitogen-activated protein kinase kinase kinase BCK1, L000000162, YJL095W
MAPKKK acting in the protein kinase C signaling pathway; the kinase C signaling pathway controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p; MAPKKK is an acronym for mitogen-activated protein (MAP) kinase kinase kinase
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Publication

Low temperature highlights the functional role of the cell wall integrity pathway in the regulation of growth in Saccharomyces cerevisiae.

Corcoles-Saez I, Ballester-Tomas L, de la Torre-Ruiz Sr MA, Prieto JA, Randez-Gil F

Unlike other stresses, the physiological significance and molecular mechanisms involved in the yeast cold response are largely unknown. Here, we show that the cell wall integrity (CWI) pathway plays an important role in the growth of S. cerevisiae at low temperatures. Cells lacking the Wsc1p membrane sensor or the MAPKs Bck1p, Mkk1p/Mkk2p or Slt2p exhibited cold sensitivity. However, there was ... [more]

Unknown Jun. 29, 2012; 0(0); [Pubmed: 22747505]

Throughput

  • Low Throughput

Ontology Terms

  • cold sensitivity (APO:0000148)

Additional Notes

  • BCK1-20 overexpression suppresses growth defect at 12 degrees of wsc1 mutant
  • BCK1-20 overexpression suppresses growth defect at 12 degrees of wsc1 wsc2 wsc3 triple mutant

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SLG1 BCK1
Dosage Rescue
Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Low-BioGRID
163705
BCK1 SLG1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-3.4627BioGRID
514898

Curated By

  • BioGRID