Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction.

Chan YW, Jeyaprakash AA, Nigg EA, Santamaria A

The KMN network (named according to the acronym for KNL1, Mis12, and Ndc80) and the more recently identified Ska complex (Ska1-3) have been shown to mediate kinetochore (KT)-microtubule (MT) attachments. How these two complexes cooperate to achieve stable end-on attachments remains unknown. In this paper, we show that Aurora B negatively regulates the localization of the Ska complex to KTs ... [more]

J. Cell Biol. Mar. 05, 2012; 196(5);563-71 [Pubmed: 22371557]

Throughput

  • Low Throughput

Additional Notes

  • figure S2C.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NDC80 SKA1
Co-localization
Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Low-BioGRID
811712

Curated By

  • BioGRID