Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2.

Hanisch A, Sillje HH, Nigg EA

Chromosome segregation during mitosis requires chromosomes to undergo bipolar attachment on spindle microtubules (MTs) and subsequent silencing of the spindle checkpoint. Here, we describe the identification and characterisation of a novel spindle and kinetochore (KT)-associated complex that is required for timely anaphase onset. The complex comprises at least two proteins, termed Ska1 (Spindle and KT Associated 1) and Ska2. Ska1 ... [more]

EMBO J. Nov. 29, 2006; 25(23);5504-15 [Pubmed: 17093495]

Throughput

  • Low Throughput

Additional Notes

  • colocalization to kinetochores

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SKA1 NDC80
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
670318

Curated By

  • BioGRID