BAIT

CIT

CRIK, STK21
citron rho-interacting serine/threonine kinase
GO Process (4)
GO Function (3)
GO Component (1)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Hrs inhibits citron kinase-mediated HIV-1 budding via its FYVE domain.

Ding J, Su L, Gao G

Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a key component of the endosomal sorting complexes required for transport and has been demonstrated to play a regulatory role in endocytosis/exocytosis and the accumulation of internal vesicles in multivesicular bodies. Citron kinase is a Ser/The kinase that we previously reported to enhance human immunodeficiency virus type 1 (HIV-1) virion production. However, ... [more]

Protein Cell Jun. 01, 2011; 2(6);470-6 [Pubmed: 21748597]

Throughput

  • Low Throughput

Curated By

  • BioGRID