BAIT

TRAF3

CAP-1, CAP1, CD40bp, CRAF1, IIAE5, LAP1
TNF receptor-associated factor 3
UPS Project
Homo sapiens
PREY

NFATC2IP

ESC2, NIP45, RAD60
nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Tumor necrosis factor receptor-associated factor (TRAF)2 represses the T helper cell type 2 response through interaction with NFAT-interacting protein (NIP45).

Lieberson R, Mowen KA, McBride KD, Leautaud V, Zhang X, Suh WK, Wu L, Glimcher LH

Recently we have identified a novel protein NIP45 (nuclear factor of activated T cells [NFAT]-interacting protein) which substantially augments interleukin (IL)-4 gene transcription. The provision of NIP45 together with NFAT and the T helper cell type 2 (Th2)-specific transcription factor c-Maf to cells normally refractory to IL-4 production, such as B cells or Th1 clones, results in substantial IL-4 secretion ... [more]

J. Exp. Med. Jul. 02, 2001; 194(1);89-98 [Pubmed: 11435475]

Throughput

  • Low Throughput

Curated By

  • BioGRID