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BAIT

DIS1

SPCC736.14

microtubule-associated protein Dis1

GO Process (3)

GO Function (1)

GO Component (7)

Gene Ontology Biological Process

attachment of mitotic spindle microtubules to kinetochore [IDA]

mitotic sister chromatid segregation [IMP]

spindle assembly involved in mitosis [TAS]

Gene Ontology Molecular Function

microtubule binding [IDA]

Gene Ontology Cellular Component

condensed nuclear chromosome kinetochore [TAS]

cytoplasm [IDA]

microtubule cytoskeleton [IDA]

mitotic spindle [IDA]

mitotic spindle pole body [IDA]

PomBase Entrez Gene RefSeq UniprotKB

Schizosaccharomyces pombe (972h)

PREY

DIS3

rrp44, SPBC26H8.10

3'-5' exoribonuclease subunit Dis3

GO Process (12)

GO Function (5)

GO Component (4)

Gene Ontology Biological Process

RNA phosphodiester bond hydrolysis, endonucleolytic [ISO]

exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [ISO]

exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [ISO]

mitotic sister chromatid segregation [IMP]

ncRNA catabolic process [ISO]

nonfunctional rRNA decay [ISO]

nuclear polyadenylation-dependent mRNA catabolic process [ISO]

nuclear polyadenylation-dependent rRNA catabolic process [ISO]

nuclear polyadenylation-dependent tRNA catabolic process [ISO]

nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay [ISO]

nuclear-transcribed mRNA catabolic process, non-stop decay [ISO]

positive regulation of Ran GTPase activity [IMP]

Gene Ontology Molecular Function

3'-5'-exoribonuclease activity [ISO]
Ran GTPase binding [IPI]
endoribonuclease activity [ISO]
protein binding [IPI]
ribonuclease activity [IMP]

Gene Ontology Cellular Component

cytoplasmic exosome (RNase complex) [ISO]

exosome (RNase complex) [IDA]

nuclear exosome (RNase complex) [ISO]

PomBase Entrez Gene RefSeq UniprotKB

Schizosaccharomyces pombe (972h)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

The fission yeast dis3+ gene encodes a 110-kDa essential protein implicated in mitotic control.

Kinoshita N, Goebl M, Yanagida M

The fission yeast mutant dis3-54 is defective in mitosis and fails in chromosome disjunction. Its phenotype is similar to that of dis2-11, a mutant with a mutation in the type 1 protein phosphatase gene. We cloned the dis3+ gene by transformation. Nucleotide sequencing predicts a coding region of 970 amino acids interrupted by a 164-bp intron at the 65th codon. ... [more]

Mol. Cell. Biol. Dec. 01, 1991; 11(12);5839-47 [Pubmed: 1944266]

Throughput

Low Throughput

Ontology Terms

phenotype: inviable (APO:0000112)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
DIS3 DIS1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Telekawa C (2018)	High	-	BioGRID	-

Curated By

BioGRID