BAIT

DSK2

L000002646, YMR276W
Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; protein abundance increases in response to DNA replication stress
UPS Project
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

MPS1

PAC8, RPK1, serine/threonine/tyrosine protein kinase MPS1, L000001698, YDL028C
Dual-specificity kinase; autophosphorylation required for function; required for spindle pole body (SPB) duplication and spindle checkpoint function; contributes to bi-orientation by promoting formation of force-generating kinetochore-microtubule attachments in meiosis I; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p; substrate of APCC(Cdh1); similar to human Mps1p
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Dosage Lethality

A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.

Publication

Ubiquitin ligase Ufd2 is required for efficient degradation of Mps1 kinase.

Liu C, van Dyk D, Choe V, Yan J, Majumder S, Costanzo M, Bao X, Boone C, Huo K, Winey M, Fisk H, Andrews B, Rao H

Ufd2 is a U-box containing ubiquitylation enzyme that promotes ubiquitin chain assembly on substrates. The physiological function of Ufd2 remains poorly understood. Here we show that ubiquitylation and degradation of the cell cycle kinase Mps1, a known target of APC E3, requires Ufd2 enzyme. Yeast cells lacking UFD2 exhibit altered chromosome stability and several spindle related phenotypes, expanding the ... [more]

Unknown Nov. 01, 2011; 0(0); [Pubmed: 22045814]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • rad23 dsk2 double mutant growth is impaired when Mps1 is overexpressed

Curated By

  • BioGRID