An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Label-free quantitative proteomics and SAINT analysis enable interactome mapping for the human Ser/Thr protein phosphatase 5.

Skarra DV, Goudreault M, Choi H, Mullin M, Nesvizhskii AI, Gingras AC, Honkanen RE

Affinity purification coupled to mass spectrometry (AP-MS) represents a powerful and proven approach for the analysis of protein-protein interactions. However, the detection of true interactions for proteins that are commonly considered background contaminants is currently a limitation of AP-MS. Here using spectral counts and the new statistical tool, Significance Analysis of INTeractome (SAINT), true interaction between the serine/threonine protein phosphatase ... [more]

Proteomics Apr. 01, 2011; 11(8);1508-16 [Pubmed: 21360678]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
STIP1 HSP90AA1	Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.	Taipale M (2014)	Low	-	BioGRID	-
STIP1 HSP90AA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Bhattacharya K (2020)	High	-	BioGRID	-
HSP90AA1 STIP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cho NH (2022)	High	-	BioGRID	3358535
STIP1 HSP90AA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cho NH (2022)	High	-	BioGRID	3372702
HSP90AA1 STIP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Gano JJ (2010)	High	-	BioGRID	818818
HSP90AA1 STIP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Hein MY (2015)	High	-	BioGRID	1451077
STIP1 HSP90AA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	0.9855	BioGRID	2243207
STIP1 HSP90AA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9735	BioGRID	3143440
HSP90AA1 STIP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021/pre-pub)	High	0.6849	BioGRID	3218493
STIP1 HSP90AA1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Taipale M (2014)	Low	-	BioGRID	-
STIP1 HSP90AA1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Bhattacharya K (2020)	Low	-	BioGRID	-
HSP90AA1 STIP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Chadli A (2010)	Low	-	BioGRID	1526786
STIP1 HSP90AA1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Johnson BD (1998)	Low	-	BioGRID	-
HSP90AA1 STIP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Mollapour M (2010)	Low	-	BioGRID	-
HSP90AA1 STIP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Mollapour M (2014)	Low	-	BioGRID	-
HSP90AA1 STIP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Sarkar S (2012)	Low	-	BioGRID	726691
HSP90AA1 STIP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Scholz GM (2001)	Low	-	BioGRID	1535015
HSP90AA1 STIP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Tsutsumi S (2012)	Low	-	BioGRID	-
HSP90AA1 STIP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Woodford MR (2016)	Low	-	BioGRID	1534827
HSP90AA1 STIP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Woodford MR (2016)	Low	-	BioGRID	-
HSP90AA1 STIP1	Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).	Wheat A (2021)	High	-	BioGRID	3681794
HSP90AA1 STIP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Brinker A (2002)	Low	-	BioGRID	-
STIP1 HSP90AA1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Chadli A (2008)	Low	-	BioGRID	1528197
STIP1 HSP90AA1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Graumann K (2000)	Low	-	BioGRID	-
STIP1 HSP90AA1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Horibe T (2011)	Low	-	BioGRID	-
HSP90AA1 STIP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Kundrat L (2010)	Low	-	BioGRID	-
HSP90AA1 STIP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Li J (2011)	Low	-	BioGRID	-
HSP90AA1 STIP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Moll A (2022)	Low	-	BioGRID	-
STIP1 HSP90AA1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Scheufler C (2000)	Low	-	BioGRID	-
STIP1 HSP90AA1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Sun L (2012)	Low	-	BioGRID	2197273

Curated By

BioGRID

Interaction Summary

HSP90AA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [TAS]ATPase activity [IDA]MHC class II protein complex binding [IDA]TPR domain binding [IDA, TAS]identical protein binding [IPI]nitric-oxide synthase regulator activity [IDA]nucleotide binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein homodimerization activity [TAS]

Gene Ontology Cellular Component

STIP1